Title: Brain dysfunction during warming is caused by oxygen limitation in larval zebrafish
Journal:
Link: https://doi.org/10.1101/2020.12.28.424529
Comments:
Spreading depolarization (SD) describes a wave in the gray matter of the central nervous system mainly characterized by inhibiting of brain electrical activity. SD is associated with the pathology of several neurological disorders. In the clinic, SD triggers the vasodilation response and activates trigemino-vascular system and has been implicated in pain sensation in migraine. Previously, researchers successfully induced SD by increasing the extracellular potassium content in brains of experimental animals. In this paper, by manipulating water oxygen levels and environment temperature, the authors induce SD in brains of larval zebrafishes to study the physiological mechanisms that limit larval zebrafish thermal tolerance. In their experiments, they raised temperature to induce SD in brains of larval zebrafishes under hypoxic environment and normal environment. The results suggested that the upper thermal limit (CTmax) appeared before the spreading depolarization, and hypoxic environment caused brain dysfunction to limit the upper thermal limit in larval zebrafish. These results provided new ideas for studying physiological mechanisms of limiting animals’ thermal tolerance.
